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One Step Smoke Check Test
SureStep One Step Smoke Check Test
About Our Test The SureStep Smoke Check Test is a lateral flow, one-step immunoassay for the qualitative detection of cotinine, the major metabolite of nicotine in human urine, at a cut-off concentration of 200 ng/ml. This product is used to obtain a visual, qualitative result and is intended for the determnination of smoking status only. Tobacco smoking results in the absorption of nicotine through the lung and buccal/nasal epithelium, after which nicotine is metabolized into about 20 metabolites excreted in urine. Cotinine, a major metabolite, accumulates in the body with regular smoking. It is reported that cotinine is stable in body fluids and has a relatively long half life of approximately 17 hours. Therefore, the detection of cotinine is less dependent on the time of sampling than that of nicotine and other metabolites. Cotinine has been widely used as a biomarker of tobacco exposure. Methods of analysis for cotinine in biological fluids include gas chromatography, gas chromatography- mass spectrometry, HPLC, HPLC-mass spectrometry, EIA and RIA. These methods usually require special equipment and complicated operation procedures.
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